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    • An acid stable trypsin-chymotrypsin inhibitor from horse gram (Dolichos biflorus)

      S L Mehta M M Simlot

      Volume 4 Issue 3 September 1982 pp 295-306

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/004/03/0295-0306

    • Keywords

       

      Trypsin-chymotrypsin inhibitor; horse gram; protease inhibitor; Dolichos biflorus

    • Abstract

       

      An inhibitor of trypsin and chymotrypsin was purified from horse gram (Dolichos biflorus) beans. The concentration of the inhibitor which provided total inhibition was 0.27 Μg/Μg tryptic enzyme and 0.46 Μg/Μg chymotryptic enzyme. The inhibitor was stable at 37‡C between pH of 3 to 11 and at 97‡C, upto pH 5.0 only. While the activities were rapidly lost in 0.1N NaO H the loss was only 5 0% in 0.1N HCl when kept for 2 h at 97‡C. On heating at pH 7.8, it remained stable upto 80‡C with a gradual loss in activities at 97‡C and a total loss occurring by autoclaving at 15 psi for 10 min. Reduction of disulphide bonds by 2-mercapto-ethanol, pronase digestion and boiling in the presence of 1 M NaCl led to reduction in the activities. However, the inhibitor was resistant to the action of pepsin and subtilisin and to urea at 37‡C.

    • Author Affiliations

       

      S L Mehta1 M M Simlot1

      1. Department of Biochemistry, Rajasthan College of Agriculture, University of Udaipur, Udaipur - 313 001

    • Dates

       
      Published
      September 1982
      Manuscript received
      14 May 1981
      Manuscript revised
      1 April 1982

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