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    • Evidence for non-coordinated synthesis of 5 S RNA in the thermophilic fungus,Thermomyces lanuginosus

      S Nageswara Rao Joseph D Cherayil

      Volume 4 Issue 2 June 1982 pp 239-244

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/004/02/0239-0244

    • Keywords

       

      5 S RNA; thermophilic fungus; ribosomal RNA; non-coordinated synthesis of PNA

    • Abstract

       

      Analysis of ribosomes and the post ribosomal supernatant fraction of actively growing cells ofThermomyces lanuginosus showed the presence of free 5 S RNA in the supernatant fraction. This 5 S RNA was identical to the ribosomal 5 S RNA in its electrophoretic mobility on 10% Polyacrylamide gel and in its base composition. 5 S RNA from both the sources gave evidence for the presence of diphosphate at the 5’ end. Most of the 5 S RNA that appeared in the cytoplasm was that transported from the nucleus during the isolation. This could be prevented by the use of a hexylene glycol-HEPES buffer.

    • Author Affiliations

       

      S Nageswara Rao1 Joseph D Cherayil1

      1. Department of Biochemistry, Indian Institute of Science, Bangalore - 560 012

    • Dates

       
      Published
      June 1982
      Manuscript received
      24 October 1981
      Manuscript revised
      3 March 1982

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