• Evidence for non-coordinated synthesis of 5 S RNA in the thermophilic fungus,Thermomyces lanuginosus

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    • Keywords


      5 S RNA; thermophilic fungus; ribosomal RNA; non-coordinated synthesis of PNA

    • Abstract


      Analysis of ribosomes and the post ribosomal supernatant fraction of actively growing cells ofThermomyces lanuginosus showed the presence of free 5 S RNA in the supernatant fraction. This 5 S RNA was identical to the ribosomal 5 S RNA in its electrophoretic mobility on 10% Polyacrylamide gel and in its base composition. 5 S RNA from both the sources gave evidence for the presence of diphosphate at the 5’ end. Most of the 5 S RNA that appeared in the cytoplasm was that transported from the nucleus during the isolation. This could be prevented by the use of a hexylene glycol-HEPES buffer.

    • Author Affiliations


      S Nageswara Rao1 Joseph D Cherayil1

      1. Department of Biochemistry, Indian Institute of Science, Bangalore - 560 012
    • Dates

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