• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/004/02/0197-0208

    • Keywords

       

      Isolation; characterization; cottonseed protein; 7S protein; gossypol

    • Abstract

       

      The major protein from glanded cottonseed has been isolated in a homogeneous form. Its S20 w value at 1 protein concentration is 6S in 1 M NaCl solution. It contains 1 carbohydrate and is free from phosphorus, gossypol (bound or free) and nucleic acid impurities. It consists of atleast seven non-identical subunits. The protein has an ultraviolet absorption maximum at 278 nm and fluorescence excitation and emission maxima at 280 nm and 325 nm respectively. Optical rotatory dispersion and circular dichroism measurements indicate that the protein consists predominantly of Β-structure and random coil. The observed near-ultraviolet circular dichroic bands can be attributed to tyrosine, phenylalanine and tryptophan residues of the protein.

    • Author Affiliations

       

      I Mohan Reddy1 A G Appu Rao1 M S Narasinga Rao1

      1. Protein Technology Discipline, Central Food Technological Research Institute, Mysore - 570 013
    • Dates

       
  • Journal of Biosciences | News

© 2017-2019 Indian Academy of Sciences, Bengaluru.