• Isolation and characterization of lysosomal alpha-mannosidase of placental tissue

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      https://www.ias.ac.in/article/fulltext/jbsc/004/02/0133-0138

    • Keywords

       

      Placental tissue acid; α-mannosidase; Concanavalin A-Sepharose chromatography

    • Abstract

       

      The acidic α-mannosidase was purified 4400-fold by affinity chromatography on Concavalin A-Sepharose and heat treatment at 65‡C in the presence of 1 mM zinc ion. The enzyme did not resolve into multiple forms as in the case of enzymes from human liver and human kidney. The pH optimum of the enzyme was 4.2 in citrate-phosphate buffer. The Km value for p-nitrophenyl-α-D-mannose was 1.9 mM. The molecular weight of the enzyme determined by gel filtration was 300,000. The enzyme contained 10.6% neutral sugars.

    • Author Affiliations

       

      Farhat A Khan1 Debkumar Basu1

      1. Neurochemistry Division, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum - 695 011
    • Dates

       
  • Journal of Biosciences | News

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