• DNA sequence organization in finger millet (Eleusine coracana)

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      https://www.ias.ac.in/article/fulltext/jbsc/003/04/0417-0430

    • Keywords

       

      DNA sequence organization; repetitive DNA; millet species

    • Abstract

       

      Approximately 39 to 49% of the genome of finger millet consists of repetitive DNA sequences which intersperse with 18% of single copy DNA sequences of 1900 nucleotide pairs. Agarose gel filtration and electrophoresis experiments have yielded the sizes of interspersed repeated sequences as 4000–4200 nucleotide pairs and 150–200 nucleotide pairs. Approximately 20% of the repeated DNA sequences (4000–4200 nucleotide pairs) are involved in long range interspersion pattern, while 60% of the repeated DNA sequences (150–200 nucleotide pairs) are involved in short period interspersion pattern.

      Based on the data available in literature and the results described here on DNA sequence organization in plants, it is proposed that plants with haploid DNA content of more than 2.5 pg exhibit mostly the short period interspersion pattern, while those with haploid DNA content of less than 2.5 pg show diverse patterns of genome organization.

    • Author Affiliations

       

      Vidya S Gupta1 P K Ranjekar1

      1. Biochemistry Division, National Chemical Laboratory, Poona - 411 008
    • Dates

       
  • Journal of Biosciences | News

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