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    • In vitro andin vivo nitrogenase activity ofRhizobium mutants and their symbiotic effectivity

      K R Dadarwal B S Kundu P Tauro

      Volume 3 Issue 2 June 1981 pp 117-123

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/003/02/0117-0123

    • Keywords

       

      Nitrogenase activity; symbiotic effectivity; inoculation; mutant strains; nodulation; nitrogen fixation

    • Abstract

       

      A slow growing nitrogen-fixing strain ofVigna radiata var.aureus (mung bean)Rhizobium which expressed nitrogenase activity in a synthetic medium was isolated from its native population. Mutants with decreased and increased nitrogenase activity were derived from this strain by treatment with acridine orange and ethidium bromide. These mutants were tested for symbiotic effectivity invivo. The effectivity of mutants with decreased nitrogenase activity in the culture medium was lower than the parent strain; however, the effectivity of mutants with higher nitrogenase activity did not increase above that of the parent. This suggests that the plant is perhaps a limiting factor in the full expression of rhizobial nitrogenase in the nodules.

    • Author Affiliations

       

      K R Dadarwal1 B S Kundu1 P Tauro1

      1. Department of Microbiology, Haryana Agricultural University, Hissar - 125 004

    • Dates

       
      Published
      June 1981
      Manuscript received
      29 September 1980
      Manuscript revised
      29 January 1981

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    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

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