• Lipid requirements for axenic cultivation ofEntamoeba histolytica

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      https://www.ias.ac.in/article/fulltext/jbsc/002/03/0235-0241

    • Keywords

       

      Entamoeba histolytica ; axenic; cholesterol; lecithin; fatty acids; trophozoite-multiplication

    • Abstract

       

      Fatty acids, cholesterol and glucose present in axenic medium are utilized by growingEntamoeba histolytica but the amoeba is unable to synthesize cholesterol from [U-14 C- ] glucose although the label is incorporated into the fatty acids and non-saponifiable fractions of the organism. Exogenously-added sonicated dispersions of cholesterol, Β-sitosterol, lanosterol, lecithin and lauric, palmitic, linoleic and stearic acids are ingested by the amoebae with subsequent loss in amoeboid movement. After a few hours the movement is regained. Cholesterol, lecithin and the fatty acids stimulate amoebic multiplication but are unable to replace serum in the medium either singly or in combination.

    • Author Affiliations

       

      N K Garg1 S R Das1

      1. Central Drug Research Institute, Lucknow - 226 001
    • Dates

       
  • Journal of Biosciences | News

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