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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/002/03/0181-0189

    • Keywords

       

      Avian glutathione-S-transferase; oxidized glutathione; 3-methylcholanthrene; phenobarbital; inhibition by oxidized glutathione; glutathione-S-transferase

    • Abstract

       

      Glutathione-S-transferase (EC 2.5.1.18) activity was assayed in hepatic and extra-hepatic tissues of pigeons using l-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene as substrates. Gluthathione-S-transferase activity towards 1-chloro-2,4-dinitrobenzene in pigeon was in the order: kidney > liver > testes > brain > lung> heart. The enzyme activity with 1-chloro-2,4-dinitrobenzene as substrate was 40–44 times higher in pigeon liver and kidney than that observed with 1,2-dichloro-4-dinitrobenzene as substrate.Km values of hepatic and renal glutathione transferase with l-chloro-2,4-dinitrobenzene as substrate were 2.5 and 3 mM respectively. Double reciprocal plots with varying reduced gluthathione concentrations resulted in biphasic curves with twoKm values (liver 0.31 mM and 4mM; kidney 0.36 mM and 1.3 mM). The enzyme activity was inhibited by oxidized gluthathione in a dose-dependent pattern. 3-Methylcholanthrene elicited about 50% induction of hepatic glutathione transferase activity whereas phénobarbital was ineffective.

    • Author Affiliations

       

      Ashwini Kumar1 M M Husain1 Hasan Mukhtar1 C R Krishna Murti1

      1. Industrial Toxicology Research Centre, Mahatma Gandhi Marg, Post Box No. 80, Lucknow - 226 001
    • Dates

       
  • Journal of Biosciences | News

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