• Interactions of progesterone with D-amino acid oxidase—different effects on apo- and holo-enzyme

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/002/01/0023-0027

    • Keywords

       

      Progesterone; D-amino acid oxidase; rat kidney; flavin adenine dinucleotide

    • Abstract

       

      A simple method using charcoal treatment was developed for the preparation of apo-D-amino acid oxidase from rat kidney homogenates. This apo-D-amino acid oxidase was used to study the effect of progesterone on the apo- and holo-enzyme. Progesterone inhibited the activity of D-amino acid oxidase, when the apo-enzyme, preincubated with saturating amounts of FAD was used; this effect varied with FAD concentration. Progesterone did not inhibit the activity when added to a mixture of non-preincubated apo-enzyme and FAD; this suggests that progesterone has different effects on apo- and holo D-amino acid oxidase.

    • Author Affiliations

       

      Faizy Ahmed1 Mahatab S Bamji1

      1. National Institute of Nutrition, Jamai-Osmania, Hyderabad - 500 007
    • Dates

       
  • Journal of Biosciences | News

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