• Isolation and characterisation of cathepsin-B from bovine pancreas

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    • Keywords


      Lysosomes; cathepsin-B; purification; bovine pancreas

    • Abstract


      A simple procedure for the isolation of cathepsin-B from bovine pancreas employing ammonium sulphate fractionation, DEAE cellulose chromatography and Sephadex G-200 gel filtration is described. The purified enzyme gave a single band on polyacrylamide gel electrophoresis. The molecular weight as determined by gel filtration of the enzyme was 26,850. ItsKm andVmax values were 12.8 mM and 0.303 Μmol/min/mg, respectively. TheKi for iodoacetamide was 0.16 mM.

    • Author Affiliations


      Renu Bansal1 J R Kidwai1

      1. Division of Biochemistry, Central Drug Research Institute, Lucknow - 226 001
    • Dates

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