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    • Isolation and characterisation of cathepsin-B from bovine pancreas

      Renu Bansal J R Kidwai

      Volume 1 Issue 4 December 1979 pp 401-407

    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/jbsc/001/04/0401-0407

    • Keywords

       

      Lysosomes; cathepsin-B; purification; bovine pancreas

    • Abstract

       

      A simple procedure for the isolation of cathepsin-B from bovine pancreas employing ammonium sulphate fractionation, DEAE cellulose chromatography and Sephadex G-200 gel filtration is described. The purified enzyme gave a single band on polyacrylamide gel electrophoresis. The molecular weight as determined by gel filtration of the enzyme was 26,850. ItsKm andVmax values were 12.8 mM and 0.303 Μmol/min/mg, respectively. TheKi for iodoacetamide was 0.16 mM.

    • Author Affiliations

       

      Renu Bansal1 J R Kidwai1

      1. Division of Biochemistry, Central Drug Research Institute, Lucknow - 226 001

    • Dates

       
      Published
      December 1979
      Manuscript received
      16 April 1979
      Manuscript revised
      28 June 1979

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