Isolation and characterisation of cathepsin-B from bovine pancreas
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A simple procedure for the isolation of cathepsin-B from bovine pancreas employing ammonium sulphate fractionation, DEAE cellulose chromatography and Sephadex G-200 gel filtration is described. The purified enzyme gave a single band on polyacrylamide gel electrophoresis. The molecular weight as determined by gel filtration of the enzyme was 26,850. ItsKm andVmax values were 12.8 mM and 0.303 Μmol/min/mg, respectively. TheKi for iodoacetamide was 0.16 mM.
Volume 48, 2023
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