• Studies with RP1 deletion plasmids: Incompatibility properties, plasmid curing and the spontaneous loss of resistance

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    • Keywords


      RP1 deletion plasmids; incompatibility; P group; plasmid curing

    • Abstract


      Four deletion plasmids, pHH301, pHH302, pHH303 and pHH401, obtained from RP1 DNA-transformed bacterial clones, were shown to be incompatible with three P plasmids inEscherichia coli K12 strains. Kinetic experiments and colony tests were used to verify the position of these R plasmids.Pseudomonas aeruginosa andE. coli strains, harbouring deletion plasmids, could be cured by using two mutagens, acriflavine and mitomycin C, which affect a percentage of the cell population. The deletion plasmid-positive strains could also be induced at an elevated temperature to spontaneously loose their plasmids.

    • Author Affiliations


      Habibul Haque1

      1. Division of Biochemistry, Department of Chemistry, Aligarh Muslim University, Aligarh - 202 001
    • Dates

  • Journal of Biosciences | News

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