• RNA synthesis in maturing avian erythrocyte nuclei

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      https://www.ias.ac.in/article/fulltext/jbsc/001/03/0335-0343

    • Keywords

       

      RNA synthesis; polyadenylated RNA; avian erythrocyte

    • Abstract

       

      The rate of RNA synthesis and its inhibition by α-amanitin in the nuclei of mature and immature avian erythrocytes are increased with the increase in ionic strength of incubation medium. Polyacrylamide gel electrophoresis indicates that heterogeneous species of RNAs are synthesised in the mature and immature erythrocyte nuclei. However, a large number of high molecular weight RNAs are synthesised in the nuclei of immature erythrocytes. Elution profiles on poly(U)-sepharose chromatography indicate that the RNAs synthesised in the nuclei of two types of cell contain poly(A) segments. Sixteen per cent of mature erythrocyte nuclear RNA syntbesised are polyadenylated, while it is 13% in immature erythrocyte nuclei. However, the total RNA synthesised is 2–3 fold higher in immature erythrocyte nuclei than that in mature erythrocyte nuclei.

    • Author Affiliations

       

      S Ganguly1 B B Biswas1

      1. Department of Biochemistry, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Calcutta - 700 009
    • Dates

       
  • Journal of Biosciences | News

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