• Isolation of sheep anterior pituitary messenger RNA and its translation in a heterologous cell-free system

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      https://www.ias.ac.in/article/fulltext/jbsc/001/03/0317-0326

    • Keywords

       

      Pituitary RNA; oligo (dT)-cellulose; prolactin-mRNA; reticulocyte ysate; immunoprecipitation

    • Abstract

       

      A preparation rich in the specific messenger RNA involved in the synthesis of prolactin from sheep anterior pituitary glands was obtained by employing both the immunochemical and affinity techniques. A dose-dependent and efficient stimulation of protein synthesis by the isolated total pituitary RNA as well as poly (A) rich RNA were achieved with the reticulocyte system. The synthesis of prolactin as one of the translational products of this cell-free system was established by specific immunoprecipitation followed by resolution on sodium dodecyl sulphate polyacrylamide gel electrophoresis.

    • Author Affiliations

       

      M S K Prasad1 2 P R Adiga1

      1. Department of Biochemistry, Indian Institute of Science, Bangalore - 560 012
      2. Department of Biological Sciences, Oakland University, Oakland, Michigan, USA
    • Dates

       
  • Journal of Biosciences | News

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