• Microbial transformation of isonicotinic acid hydrazide and isonicotinic acid bySarcina sp

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    • Keywords


      Microbial transformation; isoniazid; isonicotinic acid; Sarcina

    • Abstract


      Metabolism of isonicotinic acid and isoniazid bySarcina sp. led to the formation of two metabolites which were characterised as 2-hydroxyisonicotinic acid and citrazinic acid. The blue pigment formed during fermentation was shown to be derived from the auto-oxidation of citrazinic acid. 2-Oxo-glutarate accumulated as the major keto acid when isonicotinic acid or isonicotinic acid hydrazide metabolism was inhibited by 1 mM sodium arsenite. Isonicotinic acid, 2-hydroxy-isonicotinic acid and 2-oxo-glutarate were oxidised by isonicotinic acid hydrazide or isonicotinic acid-grown cells; citrazinic acid was, however, not oxidised. Isoniazid hydrazine hydrolase, isonicotinic acid and 2-hydroxyisonicotinic acid hydroxylases were detected in the cell-free extract ofSarcina sp. grown on isonicotinic acid hydrazide or isonicotinic acid.

    • Author Affiliations


      R C Gupta1 2 O P Shukla1 2

      1. Division of Biochemistry, Central Drug Research Institute, Lucknow - 226 001
      2. Regional Research Laboratory, Jorhat, Assam
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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