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    • Keywords


      Mitochondria; phospholipase-D; phosphatidylcholine; phosphatidylethanolamine; phosphatidylserine; swelling; adenosine triphosphatase; Ca2+ binding

    • Abstract


      Incubation of purified rat kidney mitochondrial fraction with phospholipase-D resulted in the accumulation of phosphatidic acid in the membrane due to the degradation of membrane-bound phosphatidylcholine, -serine and-ethanolamine Simultaneously with the hydrolysis of the phospholipids, cholesterol and protein were released from the mitochondrial membrane into the medium, and binding of Ca2+ by mitochondrial membranes increased. Phospholipase Dtreated mitochondrial fraction exhibited increased swellingin vitro in the early stages of incubation (15 min) after which the mitochondria were ruptured. Membrane-bound adenosine triphosphatase was partially inactivated and the enzyme activity was not significantly restored by incubation with sonicated dispersions of phosphatidylcholine,-serine and cholesterol. These results indicate that removal of choline, serine and ethanolamine from membrane-bound phospholipids disrupt phospholipid-cholesterol and phospholipid-protein association and affect functions of the membrane.

    • Author Affiliations


      S N A Zaidi1 A C Shipstone1 N K Garg1

      1. Division of Biochemistry, Central Drug Research Institute, Lucknow - 226 001
    • Dates

  • Journal of Biosciences | News

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      Posted on July 25, 2019

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