• Metal binding to pyridoxal derivatives. An NMR study of the interaction of Eu(III) with pyridoxal phosphate and pyridoxamine phosphate

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    • Keywords


      Conformation; lanthanide; nuclear magnetic resonance; pyridoxal-5′ phosphate; pyridoxamine-5′-phosphate

    • Abstract


      The solution conformations of pyridoxal-5′ -phosphate and pyridoxamine-5′-phosphate have been investigated using Eu(III) as a nuclear magnetic resonance shift probe. Binding of Eu(III) to pyridoxal phosphate results in the formation of two complexes, at the phosphate group and theo-hydroxy-aldehyde moiety, which are in slow exchange on the nuclear magnetic resonance time-scale. The lanthanide-induced pseudo contact shifts calculated using the McConnell-Robertson equation (J. Chem. Soc. (1950), 22, 1561) are in good agreement with the experimentally observed values for both pyridoxal phosphate and pyridoxamine phosphate and lead to a family of closely related conformations.

    • Author Affiliations


      B N Narasinga Rao1 C Ramakrishnan1 P Balaram1

      1. Molecular Biophysics Unit, Indian Institute of Science, Bangalore - 560 012
    • Dates

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