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https://www.ias.ac.in/article/fulltext/boms/045/0145 -
Keywords
Superoxide dismutase mimic ;Co$_3$O$_4$ nanoparticles ;CTAB@Co$_3$O$_4$@GO ;enzyme mimic study. -
Abstract
In the present study, nanocomposite (NC) of cetyl trimethyl ammonium bromide (CTAB) coated cobalt oxide nanoparticles (Co$_3$O$_4$ NPs) with graphene oxide (GO), i.e., CTAB@Co$_3$O$_4$@GO, was synthesized for superoxide dismutase mimic (SOD) activity. The NPs and NC were characterized using various analytical tools. X-ray diffraction patterns, Fourier transform infrared and scanning electron microscope–energy dispersive spectrum confirmed the presence of both GO and Co$_3$O$_4$ NPs in NC. Transmission electron microscope micrographs of NC showed GO nanosheets having CTAB-coated Co$_3$O$_4$ NPs on their surface. The NC was evaluated for SOD mimic activity using pyrogallol as a substrate. NC displayed maximum activity as compared to pristine GO and Co$_3$O$_4$ NPs. The results signified that the surfactant coating and embedding the NPs in the GO matrix helped in increasing the interaction of NC with the substrate molecules. Kinetics data was modelled using Michaelis–Menton equation. The calculated $K_m$ and $V_{max}$ values of NC were 0.0675 mM and 0.146 mol s$^{–1}$, respectively. Lower value of Michaelis constant $K_m$ as compared to the reported values, confirmits edge over other SOD mimics. Thus CTAB@Co$_3$O$_4$@GO NC holds potential for replacing natural enzyme in SOD based enzymatic assay.
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Graphical Abstract
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Author Affiliations
VAISHALI GARG1 MANPREET KAUR1 MANJEET KAUR SANGHA2 MOHAMMED JAVED3
- Department of Chemistry, Punjab Agricultural University, Ludhiana 141004, India
- Department of Biochemistry, Punjab Agricultural University, Ludhiana 141004, India
- Department of Mathematics Statistic and Physics, Punjab Agricultural University, Ludhiana 141004, India
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Dates
- Published
- 26 July 2022
- Manuscript received
- 11 November 2021
- Accepted
- 11 March 2022
- Final version published online
- 26 July 2022
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Supplementary Material
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Bulletin of Materials Science
Volume 46, 2023
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