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      https://www.ias.ac.in/article/fulltext/boms/043/0121

    • Keywords

       

      Graphene–polymer; mesoporous; Escherichia coli; biosensor; bacteria detection.

    • Abstract

       

      Here, we propose the novel fabrication of graphene–polymer (GP)-based quaternary nanocomposite and mesoporous (MS) nanomaterials sensor [NaLa(MoO$_4$)$_2$-GO-PPy (NLMG-PPy), CuZnSnSe-GO-PPy (CZSG-PPy) and In$_2$O$_3$-G-SiO$_2$ 20% (IGS20)] to address ignored challenges for Escherichia coli bacteria recognition in polluted samples.Synthesized samples were characterized through X-ray diffraction (XRD), scanning electron microscopy (SEM), energydispersive X-ray spectrometry (EDX), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), Raman spectroscopy, nitrogen adsorption–desorption isotherms, X-ray photoelectron spectroscopy (XPS) and diffuse reflectance spectroscopy (DRS). The sensor could recognize an individual E. coli cell in 1$\mu$l sample volume within 50 s. Through a low identification point of an individual cell, the MS and GP sensor had an absolute scope of 1–100 CFU per μl volume of sample (i.e. 103–105 CFU ml$^{−1}$). The high thickness of negative charge on the surface of E. coli cells actively regulates the concentration of dominant part charge carriers in the mesoporous and G-polymer monolayer, permitting an ongoing check of E. coli concentration in a known sample. Our biosensor is simple and low-cost with great selectivity and fast identification was effectively shown for E. coli detection.

    • Author Affiliations

       

      KAMRUN NAHAR FATEMA1 MD ROKON UD DOWLA BISWAS1 SEONG HO BANG2 KWANG YOUN CHO3 WON-CHUN OH1

      1. Department of Advanced Materials Science and Engineering, Hanseo University, Seosan-Si, Chungnam 356-706, Korea
      2. Department of Biological Sciences, Hanseo University, Seosan-Si, Chungnam 356-706, Korea
      3. Korea Institutes of Ceramic Engineering and Technology, Soho-Ro, Jinju-Si, Gyeongsangnam-Do, South Korea
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