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      https://www.ias.ac.in/article/fulltext/boms/042/02/0051

    • Keywords

       

      Biosensor; laccase; graphene oxide; gold–cetyltrimethylammonium bromide complex; catechol.

    • Abstract

       

      In this study, a new biosensor is developed with reliable and easy-to-use biodevice properties for catechol determination in real samples. A method is proposed for the fabrication of biosensors to sense catechol based on theadsorption method of laccase immobilization. Hence, a glassy carbon electrode was modified via graphene oxide nanosheets and then it was modified with a gold–cetyltrimethylammonium bromide nanocomposite to adsorb and immobilize laccase on the electrode surface. The results showed laccase immobilization onto the reformed glassy carbon electrode, and a direct electron transfer reaction between laccase and the electrode. The mechanism of electron transferring was EC$^{\prime}$. Also, $k_s$ and $\alpha$ were calculated as 0.41 s$^{−1}$ and 0.33, respectively. For this biosensor two linear ranges, $0.1 \times 10^{−6}$ to $5 \times 10^{−6}$ M and $16.7\times 10^{−6}$ to $166 \times 10^{−6}$ M, and a detection limit of $1.5 \times 10^{−6}$ M were obtained.

    • Author Affiliations

       

      MARYAM NAZARI1 2 SOHEILA KASHANIAN1 2 NASIM MALEKI1 NAHID SHAHABADI1 3

      1. Faculty of Chemistry, Razi University, Kermanshah 67149, Iran
      2. Nano Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah 6715847141, Iran
      3. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah 6715847141, Iran
    • Dates

       
  • Bulletin of Materials Science | News

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