• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/boms/041/06/0140

    • Keywords

       

      Amyloglucosidase; cryogel; starch hydrolysis.

    • Abstract

       

      In the present work, a newmetal-chelating platformwas designed by using IDA as a chelating agent and Cu(II) as an affinity component for amyloglucosidase adsorption. Poly(AAm-GMA) cryogels were used as structural elements, whileGMA monomer served reactive epoxy groups for IDA immobilization. Synthesized cryogels were characterized by FTIR, SEM and EDX studies. Pore diameter of the whole polymeric structure was 3–10 $\mu$m. Effects of medium pH, temperature,ionic strength along with amyloglucosidase concentrationwere also investigated for more effective amyloglucosidase adsorption and maximum adsorbed amount of amyloglucosidase was 2.93 mg g$^{−1}$ cryogel by the optimum conditions. Reusability profile of the poly(AAm-GMA)-IDA-Cu$^{2+}$ cryogels was also studied and it was found that the synthesized cryogels could be used repeatedly for many times without any significant decrease on their adsorption capacity. Also continuous hydrolysis of starch by using immobilized form of amyloglucosidase in a column system was studied.

    • Author Affiliations

       

      SINEM EVLI1 HANDE ORHAN1 PELIN SÖZEN AKTAS2 MURAT UYGUN1 3 DENIZ AKTAS UYGUN1 3

      1. Faculty of Science and Arts, Chemistry Division, Adnan Menderes University, Aydın, Turkey
      2. Faculty of Science and Arts, Chemistry Division, Celal Bayar University, Manisa, Turkey
      3. Nanotechnology Application and Research Center, Adnan Menderes University, Aydın, Turkey
    • Dates

       
  • Bulletin of Materials Science | News

    • Dr Shanti Swarup Bhatnagar for Science and Technology

      Posted on October 12, 2020

      Prof. Subi Jacob George — Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bengaluru
      Chemical Sciences 2020

      Prof. Surajit Dhara — School of Physics, University of Hyderabad, Hyderabad
      Physical Sciences 2020

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2017-2019 Indian Academy of Sciences, Bengaluru.