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    • Fulltext

       

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/boms/041/05/0134

    • Keywords

       

      Ferric oxide nanoparticles; peroxidase enzyme; enzyme mimic; CTAB.

    • Abstract

       

      In the present work, pristine and cetyl trimethyl ammonium bromide (CTAB)-coated ferric oxide nanoparticles (CTAB@Fe$_2$O$_3$ NPs) were synthesized and studied as enzyme mimics. The w/w ratio of Fe$_2$O$_3$ to CTAB was varied as 1:1 and 1:2. Transmission electron microscopic analysis revealed that pristine NPs had an average size of 50 nm, whereas the presence of CTAB resulted in the formation of nanorods with length of 130 nm. BET studies confirmed enhancement of surface area on CTAB coating, which was maximum for w/w ratio 1:1. The synthesized pristine NPs and CTAB-coatedNPs were evaluated for their peroxidase mimic activity using o-dianisidine dihydrochloride as substrate. Optimum pH, temperature, substrate and NPs concentration for the reaction were 1, 25$^{\circ}$C, 0.16 mg ml$^{−1}$ and 1 mg ml$^{−1}$, respectively. Peroxidase mimic activity of CTAB@Fe$_2$O$_3$ NPs (w/w 1:1) was higher than that of pristine NPs. However, further increasein CTAB coating (w/w 1:2) resulted in lowering of peroxidase mimic activity. Kinetic analysis was carried out at optimized conditions; maximum velocity ($V_{\rm max}$) and Michaelis constant ($K_{\rm m}$) value of CTAB@Fe$_2$O$_3$ NPs at 1:1 w/w ratio were 7.69 mM and 1.12 $\mu$mol s$^{−1}$, respectively.

    • Author Affiliations

       

      DIKSHIT GARG1 MANPREET KAUR1 SUCHETA SHARMA2 VIBHA VERMA1

      1. Department of Chemistry, Punjab Agricultural University, Ludhiana 141004, Punjab, India
      2. Department of Biochemistry, Punjab Agricultural University, Ludhiana 141004, Punjab, India

    • Dates

       
      Published
      15 October 2018
      Manuscript received
      10 May 2018
      Accepted
      20 August 2018

  • Bulletin of Materials Science | News

    • Dr Shanti Swarup Bhatnagar for Science and Technology

      Posted on October 12, 2020

      Prof. Subi Jacob George — Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bengaluru
      Chemical Sciences 2020

      Prof. Surajit Dhara — School of Physics, University of Hyderabad, Hyderabad
      Physical Sciences 2020

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

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