• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/boms/039/04/1039-1046

    • Keywords

       

      Dye affinity; Reactive Green 19; cryogel; papain.

    • Abstract

       

      In the present work, new dye ligand affinity chromatographic support for papain adsorption was synthesized. For this purpose, poly(acrylamide–methyl methacrylate) [poly(AAm–MMA)] cryogels were synthesized by using the free radical cryopolymerization technique. These cryogels were then functionalized with Reactive Green 19 dye and were characterized with Fourier transform infrared, environmental scanning electron microscopy, macroporosityand energy-dispersiveX-ray spectrometer analyses. Incorporation of the dye molecule onto cryogel structure was clearly demonstrated by these characterization techniques and pore diameter of the cryogel was found to bearound 30–40 $\mu$m. Effects of medium pH, initial papain concentration, medium temperature and ionic strength on the papain adsorption onto dye-attached cryogel were also investigated. Maximum papain adsorption was found to be 40.66 mg g$^{−1}$ cryogel by using pH 5.5 acetate buffer at 25$^{\circ}$C. Reusability profile of the cryogel was also investigatedand it was found that the adsorption capacity of the cryogel decreased only about 2.13% at the end of the 10 reuses. Activity studies of papain in desorption medium were carried out and it was found that desorbed papain wasactive and showed 88.5% of its initial activity.

    • Author Affiliations

       

      MURAT UYGUN1 RUKIYE YAVASER2 DENI Z AKTAS UYGUN2

      1. Koçarlı Vocational and Training School, Adnan Menderes University, Aydın 09010, Turkey
      2. Chemistry Department, Adnan Menderes University, Aydın 09010, Turkey
    • Dates

       
  • Bulletin of Materials Science | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2017-2019 Indian Academy of Sciences, Bengaluru.