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      https://www.ias.ac.in/article/fulltext/boms/039/02/0377-0383

    • Keywords

       

      Collagen; chitosan; biocompatibility; adsorption.

    • Abstract

       

      The aim of this study was to develop a new variant of membranes based on collagen (COL), chitosan (CHI) and alkaline phosphatase (ALP) immobilized and cross-linking with glutaraldehyde (GA) at different concentrations. The biodegradation in the presence of collagenase was investigated. Biocompatibility was evaluated by MTT assay using a mouse fibroblast cell culture type NCTC (clone 929). Non-cross-linked samples were biocompatible and membranes cross-linked with low concentrations of GA (0.04, 0.08%) were also iocompatible. However, high concentrations of GA lead to a decreased biocompatibility. The adsorption behaviour of Ca$^{2+}$ ions to all membraneswere evaluated using the Freundlich isotherms. Haemolytic studies were performed in order to consider their applications in biomineralization process. By the addition of collagen and ALP to chitosan, the haemolytic indexdecreases, the COL–CHI–ALP membrane being in the non-haemolytic domain, while the COL–CHI–ALP–GA membrane has a haemolytic index greater than 2, and is slightly haemolytic.

    • Author Affiliations

       

      E BERTEANU1 D IONITA2 M SIMOIU3 M PARASCHIV1 R TATIA1 A APATEAN1 M SIDOROFF1 L TCACENCO1

      1. National Institute of Research and Development for Biological Sciences, Bucharest 060031, Romania
      2. Politehnica Bucharest University, Faculty of Applied Chemistry and Material Science, Department of General Chemistry, Bucharest 011061, Romania
      3. National Institute of Infectious Diseases–Prof. Dr. Matei Bals, Bucharest 021105, Romania
    • Dates

       
  • Bulletin of Materials Science | News

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