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      https://www.ias.ac.in/article/fulltext/boms/031/03/0381-0386

    • Keywords

       

      Semiflexible polymers; DNA; microrheology; viscoelasticity; optical tweezers.

    • Abstract

       

      Semiflexible biopolymers play a vital role in shaping cellular structure and rigidity. In this work, we report the determination of microrheological properties of concentrated, double-stranded calf thymus DNA (CT-DNA) solutions using passive, laser-scattering based particle-tracking methodology. From power spectral analysis, we obtain dynamic shear moduli of the polymer solutions stretching over three decades of frequency (100–103 Hz) and over concentration ranges spanning from very dilute to concentrated regime. We also study the effects of altered ionic strength and denaturation on the shear modulus. Our results indicate that (CT-DNA) exhibits predominantly elastic behaviour in the concentration range we probed. From the measurements of the plateau shear modulus, 𝐺p, we conclude that DNA generally behaves like a semiflexible polymer in a good solvent even at low ionic strength. We have thus demonstrated application of passive microrheological method using optical tweezers to DNA solutions. Further extensions of the technique and its applications are discussed.

    • Author Affiliations

       

      Arun S Rajkumar1 B M Jaffar Ali1

      1. Life Sciences Division, AU-KBC Research Centre, MIT, Anna University, Chennai 600 044, India
    • Dates

       
  • Bulletin of Materials Science | News

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