Fine structure of the regional differentiation of the ductus ejaculatorius simplex (EJ1, EJ2, EJ3) along with the role of their secretions in sperm activation and motility in two Noctuid species,Heliothis armigera (Hübner) andSpodoptera litura (Fabricius) (Lepidoptera). I. Fine structure and function of simplex 1 (EJ1)
The common unpaired ejaculatory duct or simplex of male reproductive tract ofHeliothis armigera andSpodoptera litura, on the basis of morphology and anatomy, are broadly distinguished into EJ1, EJ2 and EJ3 regions. The proximal region (EJ1) of common duct to the ductus ejaculatorius duplex characteristically contains transparent elastic gelatinous secretion which is capable of sperm activationin vitro. The immotile spermatozoa within the duplex are induced-in vitro activated by diluting the same to 0·15 M in HEPES-KOH buffer, maintaining pH at 7. The activation is facilitated by the addition on 10mg/ml bovine serum albumin and activation is triggered by an addition of a small fraction of activator substance taken from EJ2. EJ1 of both species exhibit only a single layered epithelium. Merocrine and apocrine are the mode of secretion occurring inHeliothis armigera while merocrine is the only mode of secretion occurring in EJ1 ofSpodoptera litura.