In vitro studies on uptake, storage and disappearance of norepinephrine were conducted on spleen slices of chicken. Uptake process was found to be time and concentration dependent. Maximum uptake of norepinephrine occurred at 10−4 M concentration after 45 min of incubation. Cocaine, metanephrine, phenoxybenzamine and a-combination of cocaine and metanephrine, all inhibited the accumulation of norepinephrine, the last being most effective, indicating that both uptake, and uptake, processes are operative simultaneously and independently in disposition of amine. Disappearance studies following maximum accumulation indicated that the release was monophasic from a single pool. Reserpine pretreatment significantly reduced the accumulation and enhanced the release of norepinephrine.