Histochemical demonstration of esterase in certain freshwater larval trematodes with a note on neuroanatomy
Histochemical method for non-specific esterases has been applied to cercariae belonging to 4 different trematode groups viz Monostome, Amphistome, Xiphidio and Echinostome. Various structures containing esterases revealed positive reactions to the standard method and are visualized in simple whole mount preparation. Different degrees of non-specific esterase activities were observed in the nervous and the excretory systems, suckers and sub-tegumentary cells. In general, nervous systems of cercariae are composed of a pair of cerebral ganglia connected to each other by a transverse commissure to form a cerebral complex. These give off 3 pairs of anterior longitudinal and 3 pairs of posterior longitudinal nerve cords. Simultaneously nervous systems of redia of monostome cercaria have also been focused in the present study.