University of Hyderabad, Hyderabad
Siddavattan Dayananda is presently a Professor in the Department of Animal Sciences, School of Life Sciences at University of Hyderabad. His group works in the areas of horizontal gene transfer among soil bacteria, transport of prefolded proteins across bacterial membrane and the development of bioremediation strategies useful in leather processing, insect control and detection and decontamination of nerve agents. He is a receipient of many national and international fellowships and awards inlcuding the DAAD Fellowship (1988- 90), Commonwealth Academic Staff Fellowship (1995–1996), The Wellcome Trust, UK International Research Development Award and the Andhra Pradesh Scientist Award-2008 of the AP State Council of Science and Technology. He is a Fellow of all the three Science Academies in India. He was elected as a Fellow of the Indian Academy of Sciences in 2018.
SESSION 2D: Inaugural lectures by Fellows/Associates
R K Srivastava, BHU, Varanasi
Organophosphate hydrolase exists as part of Ton complex in Sphingobium fuliginis ATCC27551 and plays a role in active transport of nutrients across energy deprived outer membrane
In soil bacterium Sphingobium fuliginis, organophosphate hydrolase (OPH) exists as part of Ton complex comprising of TonB, ExbB/ExbD. The OPH physically interacts with both ExbD and TonB. The surface exposed arginine residues (R91 and R96) of OPH facilitate its physical interaction with ExbD. A functional Ton complex forms in heterologous systems only when OPH is co-expressed with ExbB and ExbD. The OPH hydrolyzes the lactone ring present in enterobactin and facilitates the release of iron strongly bound to enterobactin. The Sphingobium fuliginis TonB dependent transport (SfTonBDT) system complemented E. coli TonBDT null mutant. The cells having SfTon complex with OPH showed better growth and enhanced iron uptake when compared to a similar strain generated without OPH. The study by the speaker and his group reveals existence of novel TonB dependent transport system in S. fuliginis ATCC 27551.