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Table of contents

Japanese encephalitis vaccine: moving away from mouse brain

Japanese encephalitis

Incidence of Japanese encephalitis

Japanese encephalitis in India

Japanese encephalitis vaccine

What is required in an ideal JEV vaccine ?

Japanese Encephalitis Virus Genome and Proteins

JEV Structural Proteins : Properties and Functions

JEV vaccine development at National Institute of Immunology

Peptide vaccine against Japanese encephalitis

Peptide vaccine : promises and pitfalls

Johnson grass mosaic virus

Self assembly of Potyvirus coat protein

Potato virus Y coat protein

Slide 15

JGMV coat protein

Synthesis of JGMV Coat protein in E. coli

Predicted secondary structure of the JGMV coat protein RNA

High level expression of the modified JGMV coat protein gene in E. coli

Virus-like-particles formed by Johnson grass mosaic virus coat protein in E. coli

Purification of JGMV VLPs

Structure of flavivirus envelope protein

JEV peptide sequences chosen for presentation on recombinant VLPs

JGMV fusion protein synthesis in E. coli

JGMV fusion protein synthesis in E. coli

VLP formation in E. coli by JGMV CP-A

Synthesis of JGMV CPL-A in E. coli

JGMV fusion protein Synthesis in E. coli

Mice immunizations

Anti-peptide antibody response in mice

Anti-E antibody response in mice

Endpoint ELISA titers of mice sera

Immunofluorescence on JEV-infected cells by the mouse sera

JEV neutralization by sera from mice immunized with the JEV peptide-fused-CP

Mouse survival after challenge

JEV peptide vaccine

Development of a plant-derived oral JEV vaccine

Synthesis of JEV E protein in E. coli

Mice immunization

Generation of anti-JEV antibodies in mice fed with JEV E protein

Generation of cellular immunity against JEV in mice fed with JEV E protein

Oral feeding with Japanese encephalitis virus envelope (E) protein induces anti-JEV immune responses in mice

Plant-derived JEV vaccine

Japanese encephalitis vaccine : moving away from mouse brain

ACKNOWLEDGEMENTS

Author: OpenOfficeOrg1.1.3