Determination of genetic relationships among elite thermosensitive genic male sterile lines (TGMS) of rice (Oryza sativa L.) employing morphological and simple sequence repeat (SSR) markers
Vikas Kumar Singh Priti Upadhyay Pallavi Sinha Ashish Kumar Mall Sanjay Kumar Jaiswal Atul Singh Ranjith Kumar Ellur Sunil Biradar R. M. Sundaram Sukhpal Singh Ilyas Ahmed B. Mishra A. K. Singh C. Kole
rice; SSR markers; thermosensitive genic male sterility; morphological traits; genetic diversity.
A set of morphological traits and SSR markers were used to determine the genetic relationship among 12 elite thermosensitive genic male sterile (TGMS) lines developed at three different research institutions of India. Agro-morphological data recorded on 20 morphological traits revealed a wide base of genetic variation and a set of four morphological traits could distinguish most of the TGMS lines. Analysis with 30 SSR markers (20 EST-SSRs and 10 genomic SSRs) revealed 27 markers to be polymorphic, amplifying a total of 83 alleles. Each SSR marker amplified 2–6 alleles with an average of 2.76 alleles per marker and a PIC value varying from 0.54 to 0.96. Cluster analysis based on SSR and morphological data clearly differentiated the lines according to their source of origin. Correlation analysis between morphological and molecular data revealed a very poor association ($r = 0.06$), which could be attributed to selection pressure, genetic drift, sampling error and unknown relationship among related lines. The SSR markers discriminated the genotypes distinctly and quantified the genetic diversity precisely among the TGMS lines. Data on the yield per plant indicated that the genotypes grouping under a similar cluster showed same heterotic behaviour as compared to the genotypes from different clusters when crossed to similar pollinators.
Vikas Kumar Singh1 Priti Upadhyay1 Pallavi Sinha2 Ashish Kumar Mall2 Sanjay Kumar Jaiswal1 Atul Singh2 Ranjith Kumar Ellur2 Sunil Biradar3 R. M. Sundaram3 Sukhpal Singh3 Ilyas Ahmed3 B. Mishra3 A. K. Singh2 C. Kole4