• Activity, stability and kinetic parameters for 𝛼-chymotrypsin catalysed reactions in AOT/isooctane reverse micelles with nonionic and zwitterionic mixed surfactants

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    • Keywords


      𝛼−Chymotrypsin; stability; 𝑝-nitrophenyl acetate; zwitterionic surfactants; reverse micelles

    • Abstract


      Reverse micelles (RMs) of sodium 1,4-bis(2-ethylhexyl)sulphosuccinate (AOT) in nonpolar organic solvents are widely known to have very high solubilization power for water. The method is applied to the hydrolysis of 𝑝-nitrophenyl acetate (PNPA) catalysed by 𝛼-chymotrypsin (𝛼-CT) in AOT/isooctane/buffer RMs. The increase in 𝛼-CT activity and stability was an optimum at wo ([H2O]/[AOT]) = 10, z [Isooctane]/[AOT]) = 5. Three typical surfactants were selected based on their head group charges: a non-ionic surfactant Triton-X 100 and two zwitterionic sulphobetaine surfactants of the type C𝑛H2𝑛+1N+Me2 (CH2)3 SO$^{−}_{3}$ (n = 10; SB3-10, n = 16; SB3-16). The kinetic parameters (such as 𝑘cat and 𝐾M) of the 𝛼-CT at 27°C were determined and compared in the absence and presence of three surfactants. The effect of chain length of zwitterionic surfactant (SB3-10 and SB3-16) on the enzymatic efficacy of 𝛼-CT as a function of mixed surfactant addition has been investigated in AOT/isooctane RMs at pH 7.75.

    • Author Affiliations


      Santosh Kumar Verma1 Kallol K Ghosh2

      1. Shri Shankaracharya Institute of Technology and Management, Bhilai 490 020, India
      2. School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur 492 010, India
    • Dates

  • Journal of Chemical Sciences | News

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