• The amino-terminal domain of human signal transducers and activators of transcription 1: Overexpression, purification and characterization

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    • Keywords


      Affinity chromatography; amino-terminal domain; SDS-PAGE electrophoresis; STAT proteins

    • Abstract


      The dual functional signal transducers and activators of transcription (STAT) proteins are latent cytoplasmic transcription factors that play crucial roles in host defense. Animals that lack these proteins are highly susceptible to microbial and viral infections and chemically induced primary tumours. We have over expressed the aminoterminal domain of human STAT1 (hSTAT1) in Escherichia coli and purified it by affinity chromatography and gel filtration chromatography. The entire process has been monitored by gel electrophoresis. The pure protein has been characterized by mass spectrometry and 2-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy. Our results indicate that the N-terminus of hSTAT1 exists as a dimer in solution.

    • Author Affiliations


      Arati Prabhu1 Evans Coutinho1 Sudha Srivastava2

      1. Department of Pharmaceutical Chemistry, Bombay College of Pharmacy, Kalina, Mumbai 400 098, India
      2. Tata Institute of Fundamental Research, Homi Bhabha Road, Navy Nagar, Colaba, Mumbai 400 005, India
    • Dates

  • Journal of Biosciences | News

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